Doing a frequency count for a set of sequences:

1. Create a list to hold the frequency count.
2. For each sequence database from which you wish to count calls, from the main menu select 'Parse' followed by 'Parse several sequences'. Select the database and range of records to process. Accept the default output file (\pallseq.out). Select the list created above in step 1. Select 'No' for 'Zero all use counts in list?'.
3. The program then parses the sequences selected and updates the list. Delete the output file.
4. Repeat step 2 and 3 until all sequences have been processed.
5. View or print the list.
   • To view the list: From the main menu, select 'List' followed by 'View' and select the list created above in step 1. After the list is displayed, select 'Display' then 'Change Use count range' and set the minimum to 0 and the maximum to 9999. Then select 'Display' then 'L-H side' then 'Use count'. Then select 'Display' then 'Sort by' then 'Descending use count'.
   • To print the list: From the main menu, select 'List' followed by 'Print a list'. Select the list created in above in step 1. Select the 'Frequency count' option, and decide whether to send the output to the printer or to a text file. Click on the 'Print' button.